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KMID : 0380620100420060733
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Korean Journal of Food Science and Technology
2010 Volume.42 No. 6 p.733 ~ p.738
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Optimization of Ethanol Extraction Conditions for Antioxidants from Zizyphus jujuba Mill. Leaves Using Response Surface Methodology
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Min Dul-Lae
Lim Seok-Won
Ahn Jun-Bae
Choi Young-Jin
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Abstract
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The leaves of Zizyphus jujuba have been used for various purposes including medicine and nutrition. In this study, the conditions for the ethanol extraction of antioxidant from Zizyphus jujuba were optimized using response surface methodology (RSM). A Box-Behnken design containing 15 experimental runs with three replicates was employed to study the effects of solvent extraction conditions such as extraction temperature (oC, X1), extraction time (min, X2), and ethanol concentration (%, X3) on the extraction yield of antioxidants from Zizyphus jujuba. The yields of total polyphenols and total flavonoid, and electron donating activity (EDA) were considered as response variables. The second-order polynomial model gave a satisfactory description of the experimental results showing different patterns of extraction conditions with variation in the linear, quadratic, and interaction effects of the independent variables. Based on four-dimensional RSM, one of the optimized sets of conditions was 45% ethanol, 45oC, and an extraction time of 15 min. Under the optimal conditions, the predicted values were 177.64 mg/g dry basis, 35.99 mg/g dry basis, and 86.14% Vit.C equivalents for total polyphenols, total flavonoids, and EDA, respectively. The experimental values showed good agreements with the predicted values.
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KEYWORD
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Zizyphus jujuba, antioxidant, extraction, response surface methodology
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